The sodium salt of di (diethylamino) fluorescein, often referred to as the sodium salt of di (diethylamino) fluorescein, has a wide range of main uses.
In the field of medical testing, this is an important fluorescent labeling reagent. In immunofluorescence detection technology, it can bind to a specific antibody. By chemical reaction, the active group on the sodium salt is covalently linked to some groups on the antibody. In this way, when a specific antibody binds specifically to the antigen in the sample to be detected, with its own excited fluorescence emission characteristics, the location and distribution of the antigen can be clearly observed with the assistance of fluorescence microscopy or flow cytometry equipment, thereby assisting in the diagnosis and monitoring of diseases. For example, when detecting diseases caused by certain viral infections, this labeled antibody can be used to locate viral antigens, providing a key basis for accurate diagnosis.
In the field of biological imaging, it is also a commonly used fluorescent probe. Many biomolecules or cell structures in organisms have weak self-fluorescence and are difficult to directly observe. The sodium salt of bis (diethylamino) fluorescein can specifically interact and bind to certain biomolecules according to their structural characteristics. For example, after binding to specific proteins and nucleic acids in cells, it is excited by light at a suitable wavelength to generate a strong fluorescent signal. Researchers can use this to track the dynamic changes of these biomolecules in living cells or organisms in real time, such as protein transportation, nucleic acid transcription, etc., providing a powerful tool for exploring the mysteries of life activities.
In terms of environmental monitoring, it can be used to detect specific pollutants. Certain heavy metal ions or organic pollutants can react with the sodium salt, causing its fluorescence characteristics to change. Qualitative and quantitative analysis of these pollutants in the environment can be achieved by detecting changes in parameters such as fluorescence intensity and wavelength. For example, when detecting trace heavy metal mercury ions in water, the mercury ions react with bis (diethylamino) fluorescein sodium salt, which will significantly reduce the fluorescence intensity. Based on this change, the content of mercury ions in water can be determined to protect the safety of the ecological environment.

(Diethylamino) ethyl silicate, also known as diethylaminoethoxysilane, is a chemical substance that is very important in the chemical industry. It has the following physical properties:
Looking at its properties, it is a colorless and transparent liquid under normal conditions, with a pure texture, no impurities visible to the naked eye, and good flow. This property makes it easy to control and handle in many chemical operations, such as dumping, mixing, etc.
Smell its smell, which has a special pungent smell. However, it should be noted that although this smell has certain characteristics, it also implies that it may have irritating effects on the human respiratory tract, eyes and nose, etc., and protective measures should be taken during use.
When it comes to solubility, (diethylamino) ethyl silicate is soluble in most organic solvents, such as ethanol, ether, toluene, etc. This good solubility allows it to be uniformly mixed with other organic components in the fields of organic synthesis and coating preparation, so as to better exert its chemical properties and achieve the desired reaction or function.
Its density is slightly smaller than that of water, about 0.93 - 0.97g/cm ³. This density characteristic is of great significance when it involves delamination, separation, etc. For example, in some reaction systems, the product can be effectively separated and purified according to its density difference with water or other liquids.
The boiling point is between 160 - 170 ° C. This boiling point condition determines the temperature range required for heating, distillation, etc. By precisely controlling the temperature, the separation and concentration of (diethylamino) ethyl silicate can be achieved to meet the needs of different production processes. These physical properties of
(diethylamino) ethyl silicate play a crucial role in its application in chemical production, material preparation and many other fields. Only by deeply understanding and mastering these properties can it be used more scientifically, rationally and safely.

Sodium di (hydroxyethyl) aminoethanesulfonate has mild properties and buffering ability. In biochemical experiments, the acid and base of the stable system, such as cell culture, protein analysis, etc., all rely on it to adjust the pH value to ensure the right environment and help the reaction.
This agent has good solubility, is easily soluble in water, can quickly disperse in the liquid phase, and becomes a uniform state, which is convenient for experimental preparation. And chemically stable, under normal temperature, it is not easy to cause qualitative changes, and can be stored for a long time without losing its properties, providing reliable quality for experiments.
Furthermore, it has a good affinity with biomolecules, has little effect on proteins, nucleic acids, etc., and does not disturb their structure and function. Therefore, it is commonly used in the study of cells, enzymes, etc., which can reduce the dryness of biological systems and make the results near true.
It has low toxicity. Compared with other reagents, it is less toxic to organisms. When experimenters operate, they should be safe and worry less. In the fields of biomedicine, pharmaceutical research and development, etc., which require contact with biological materials, it is widely used, which not only guarantees the experimental effect, but also takes into account the safety.

To prepare 3- (diethylamino) benzaldehyde oxime, there are various methods. First, it can be obtained by reacting 3- (diethylamino) benzaldehyde with hydroxylamine hydrochloride in an appropriate solvent. First take an appropriate amount of 3- (diethylamino) benzaldehyde, place it in a reactor, use ethanol as a solvent, stir to dissolve it. In addition, hydroxylamine hydrochloride is dissolved in an appropriate amount of water, slowly dripped into the kettle, and at the same time control the temperature to ensure that the reaction is gentle and orderly. After dripping, keep stirring, and when the reaction reaches the desired level, it can be monitored by thin chromatography. After the reaction, pour the mixture into the separation funnel, extract it with organic reagents, separate the organic phase, and then wash and dry it with water to remove impurities. After distillation under reduced pressure, pure 3- (diethylamino) benzaldehyde oxime was obtained.
Second, with 3- (diethylamino) benzoic acid as the starting material, 3- (diethylamino) benzyl alcohol was first obtained by reduction reaction, and then oxidized to 3- (diethylamino) benzaldehyde, and finally reacted with hydroxylamine to form the target product. During reduction, a strong reducing agent such as lithium aluminum hydride can be selected and carried out in a solvent such as anhydrous ethyl ether. After obtaining 3- (diethylamino) benzyl alcohol, it is oxidized to 3- (diethylamino) benzaldehyde under suitable conditions with an appropriate oxidizing agent such as manganese dioxide. Then, 3 - (diethylamino) benzaldehyde oxime can be obtained by reacting with hydroxylamine hydrochloride in the presence of alkali. During the reaction process, each step needs to be strictly controlled by temperature and time to improve the yield and purity.

3 - (divinylamino) silane coupling agent has the following precautions during use:
First, it is related to storage. This agent should be stored in a cool, dry and well-ventilated place, away from fire and heat sources. Because of its volatility and flammability, if stored improperly, it is easy to cause fire or even explosion in case of open flame or high temperature. And it should be strictly sealed to prevent it from reacting with moisture, oxygen, etc. in the air and causing its performance to deteriorate.
Second, the operating environment is critical. The use site must be well ventilated to prevent its volatile gases from accumulating in the air. After reaching a certain concentration, it will not only irritate the respiratory tract, eyes and other parts of the human body, but may also form explosive mixed gases. If conditions permit, it is best to operate in a fume hood.
Third, it involves protective measures. Users must take good personal protection and wear suitable protective gloves to avoid direct contact between the agent and the skin, because it may cause irritation and corrosion to the skin. At the same time, wear protective glasses to prevent the agent from splashing into the eyes. If it is accidentally splashed, it should be rinsed with a lot of water immediately and seek medical attention as soon as possible.
Fourth, in terms of dosage. The dose should be precisely controlled according to the specific purpose and requirements of use. If the dosage is too small, the expected coupling effect may not be achieved; if the dosage is too large, it will not only cause waste, but also may have adverse effects on the performance of subsequent products.
Fifth, about mixing and compatibility. When mixed with other substances, it is necessary to know its compatibility with other ingredients in advance. In some cases, improper mixing may cause chemical reactions, resulting in changes in system performance, and even produce adverse phenomena such as precipitation and gel.
Sixth, for the use of equipment. After use, the relevant equipment should be cleaned in time to prevent the residue of chemicals, so as not to corrode the equipment and affect the service life of the equipment and the effect of the next use.